Summary Other designations. We have altered the codons of the wild type TetA gene, optimizing and de-optimizing several segments of the coding sequence. These domains mainly consist of a helix-turn-helix (HTH) motif that is common in TetR protein family members (see below). The results of tetA gene sequencing procedure. 2018 Dec 27. pii: 5264291. doi: 10.1093/nar/gky1286. Gene provides a unified query environment for genes defined by sequence and/or in NCBI's Map Viewer. The mutated tetA gene was detected in a wide range of bacteria associated with mobile genetic elements ( Table 3 ) [23] , [24] , [25] . Isolates from the lagoon that carried tetA, tetC, and sul3 genes were significantly more prevalent during spring (63.3–96.7%) than during winter (13.1–67.8%). In the presence of tetracycline, TetR no longer represses. (HM453327.1), Klebsiella sp. TetA. DNA sequence analysis of an 8.0-kb region revealed a novel gene [tetA(41)] that was predicted to encode a tetracycline efflux pump. Phylogenetic analysis showed that the TetA(41) protein was most closely related to the Tet(39) efflux protein of Acinetobacter spp. tetA-like gene (EMBL accession number HG424699) was identified in the whole-genome sequence of S. thermophilus DSM 20617 T. Pure genomic DNA was extracted and purified using the UltraClean™ Microbial DNA Isolation Kit (MO BIO, CA). Each TetA variant is coupled with a distinctive fluorescent gene, and each pair of genes is flanked by lox sites. GeneRIFs: Gene References Into Functions. Furthermore, the frequency of isolates that contain genes coding for aadA and tetB was significantly greater in soil samples (82.6–97.2%) when compared to swine manure (16.8–86.1%). The tetracycline resistance genes (including tetA and tetB) have been detected from clinical and non-clinical samples The DNA sequence of the entire tetA gene in the resulting construct, pSTL361, containing a short deletion marked with a NotI restriction site, was confirmed. The DNA-binding domains of TetR recognize a 15 base pair palindromic sequence of the TetA operator. Plasmid × Plasmid Recombination Assays. The purified PCR product was submitted to the showed a 100% similarity with other reported tetA genes Macrogen Genomic Division (Korea) and nucleotide sequencing of Aeromonas sp. yet had less than 80% amino acid identity with known tetracycline efflux pumps. Plasmid KO701: pMVP (L3-L2) HA tag + pA; EF1a::TETa-pA from Dr. Christopher Newgard's lab contains the insert HA epitope tag-polyA + EF1a::TETa-polyA and is published in Nucleic Acids Res. tetA TetA [] Gene ID: 2716475, discontinued on 1-Aug-2020. The sequence of the mutated tetA gene is more commonly deposited to GenBank than the tetA gene from plasmid RP1, indicating the predominance of mutated tetA in tetracycline-resistant bacteria. This plasmid is available through Addgene. Thus, these constructs place the fliC gene under the control of the tetA promoter, which is induced by the addition of tetracycline. The most common gene detected in tetracycline-resistant isolates from 2010 to 2011 was tetA (81.3%), while tetB was most often (86.5%) found in isolates from 2005 to 2006 . The tetA gene product confers resistance to tetracycline and is repressed by the TetR repressor.